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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: ZNStress: a high-throughput drug screening protocol for identification of compounds modulating neuronal stress in the transgenic mutant sod1G93R zebrafish model of amyotrophic lateral sclerosis

Fig. 4

Representative SSMD* scores of a plate and screen replicate from the ZNStress assay. a The plate comprising 12 positive controls (riluzole, black dots) and 12 negative control (DMSO, black triangles) and 48 test compounds (grey squares) were calculated for SSMD*. Riluzole shows a clear inhibitory effect and consistently shows a negative SSMD*, while most test compounds and DMSO controls show very little inhibitory activity. b 2000 compounds from the MicroSource Spectrum library were tested over 40 plates. Positive control for inhibitor of neuronal stress (riluzole, grey triangle) and negative DMSO controls (black circle) and test compounds (grey circles). A small fraction of drugs in the screen show biological effects comparable to riluzole and a small fraction of compounds in the library also showed positive SSMD values above 2, indicating that they were activators. Compounds that showed similar efficacy in two trials were classified as hits

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