Table 1 Antibodies used in western-blot (WB) and immunohistochemical (IHC) analyses

rabbit anti-ataxin-7

Thermo Scientific/(PA1-749)

1:2000

1:5000

rabbit-anti-ubiquitin

Dako/(Z0458)

–

1:2000

mouse anti-ataxin-7 (1C1)

Provided by Dr Didier Devys

1:5000

1:5000

mouse anti-Calbindin D-28 K

Swant/(300)

–

1:4000

mouse anti-microtubule-associated Protein 2 (MAP2)

Sigma-Aldrich/(M1406)

–

1:2000

mouse anti-synaptophysin

Abcam/(ab32594)

–

1:250

mouse anti-SNAP-25

Abcam/(ab24737-250)

–

1:1000

rabbit-anti-PSD-95

Abcam/(ab18258)

–

1:1000

rabbit anti-Glial Fibrillary Acidic Protein (GFAP)

Dako/(Z0334)

–

1:2000

rabbit anti-ionized calcium binding adapter molecule 1 (Iba1)

Wako/(019-19741)

–

1:3000

rat anti-CD11b/Mac-1

AbD Serotec/(MCA74G)

–

1:1000

rabbit anti-GFP

Abcam/(ab6556)

–

1:5000

rabbit cleaved caspase-3 (Asp175)

Cell Signaling/(9664S)

–

1:500

mouse anti-neurofilament 70 KDa

Millipore/(MAB1615)

–

1:1000

rabbit anti-phospho-TDP-43 (pS409/410)

Cosmo Bio/(CAC-TIP-PTD-P01)

1:1000

1:3000

rabbit anti-TARDBP (TAR DNA-binding protein 43)

Proteintech/(10782-2-AP)

–

1:3000

Mouse anti-FUS/TLS(4H11) (Fused in Sarcoma/Translocated in Sarcoma)

Santa Cruz/(sc-47711)

1:1000

1:500

rabbit-anti-MBNL1 (Muscleblind-like protein 1)

Santa Cruz/(sc-47740)

1:1000

1:500

rabbit-anti-MBNL2 (Muscleblind-like protein 2)

Abcam (ab171551)

1:2000

1:500

rabbit-anti-MBNL2

Abcam (ab108519)

–

1:500

rabbit anti-tubulin

Abcam/(ab134185)

1:5000

–

rabbit anti-iNOS (Nitric oxide synthase)

Cayman Chemicals/(160862)

–

1:400

rat anti-CD68

Abd serotec/(MCA1957)

–

1:500

rabbit anti-TGF-ß (Transforming growth factor beta)

Abcam/(ab66043)

–

1:1000

rabbit anti-TREM2 (Triggering receptor expressed on myeloid cells 2)

R&D systems/(MAB1729)

–

1:500

goat anti-vimentin

Santa Cruz/(sc-7557)

–

1:500

rabbit anti-p62/SQSTM1 (sequestosome 1)

BD Biosciences/(610832)

–

1:500

mouse anti-actin

Abcam/(ab9484)

1:4000

–

mouse anti-SMI-25

Covance/(SMI-25R)

–

1:2000