Fig. 3

AD-associated changes in the levels of BIN1 isoforms. a Immunoblot analysis of BIN1 and Amphiphysin 1 levels in the brain samples from controls and AD cases. The age of each individual is listed. The blots were simultaneously probed with BIN1 and Amphiphysin 1 antibodies. The levels of actin were used as the control for loading. Note that the gray and white matter blots were scanned using different conditions to achieve equivalent signals. b The levels of Amphiphysin 1 and BIN1 isoforms in the gray matter were quantified using the Li-COR system and normalized to the levels of actin. The relative expression levels of Amphiphysin 1 and BIN1: H (~90 kDa) were plotted as mean ± SEM (n = 5). The statistical significance was analyzed by ANOVA. c The plot shows the percentage of BIN1:L (~65-75 kDa) isoform relative to all BIN1 isoforms in the gray matter of each tissue sample. d Pearson r correlation matrix analysis. The levels of multiple cellular markers were quantified as described above and the relative expression level differences in AD samples relative to the controls were used to generate the correlation matrix. Larger blue-colored circles represent a higher degree of correlation, and larger red-colored circles represent an inverse correlation. Smaller neutral-colored circles represent a lack of correlation. A color scale is drawn at the bottom. GM = gray matter; WM = white matter; Amph 1 = Amphiphysin 1