Fig. 7

Expression of BIN1 in mature oligodendrocytes. BIN1 immunolabeling is in green and the marker labeling is in red. Nuclear staining with Hoechst is in blue. a-c Sagittal sections of the cerebellum were labeled with the indicated antibodies. BIN1 labeling is prominent in the white matter whereas no labeling for Amphiphysin was observed in the white matter. Synaptophysin and Amphiphysin 1 labeling are prominent in the molecular layer synaptic terminals and in the granule cell layer mossy fibers. Higher magnification of the granule cell layer reveals BIN1-positive processes (arrows) around synaptophysin-positive mossy fibers c. The areas indicated by the rectangles are enlarged on the right. Line-scan analysis of BIN1 and synaptophysin fluorescence intensities plotted as graphs shows that the proteins localize to distinct structures. d BIN1 labeling in the cortex is found along the internodal segments of processes (arrows) adjacent to the paranodal localization of Caspr. e and f All BIN1-positive cells (arrows) are also positive for ASPA and TPPP, which are markers of mature oligodendrocytes. A higher magnification of the boxed areas is shown. g–j BIN labeling in cortex and corpus callosum (white arrows) does not overlap with NG2-labeled OPCs, Iba1-labeled microglia or GFAP-labeled astrocytes (yellow arrows). Syn = synaptophysin; Amph 1 = Amphiphysin 1; WM = white matter; ML = molecular layer; GCL = granule cell layer; CC = corpus callosum; CB = cingulum bundle