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Fig. 8 | Molecular Neurodegeneration

Fig. 8

From: Predominant expression of Alzheimer’s disease-associated BIN1 in mature oligodendrocytes and localization to white matter tracts

Fig. 8

Immunoblot analysis of BIN1 expression in rodent brain and cultured oligodendrocytes. a The post-natal increase in BIN1 expression. Immunoblots of brain lysates from the indicated developmental periods were analyzed by Western blots using the indicated antibodies. The levels of flotillin 2 and actin were assessed as loading controls. The graph represents quantification of BIN1 signal intensities normalized to actin. b The increase of BIN1 expression during in vitro oligodendrocyte differentiation. Lysates of cultured rat OPCs, mature oligodendrocytes (OL) following 3 or 5 days of differentiation, mature neurons, and astrocytes were analyzed by immunoblots. The levels of MBP, CamKII, and GFAP were analyzed as markers for mature oligodendrocytes, neurons, and astrocytes, respectively. BIN1 is highly expressed in mature oligodendrocytes whereas Amphiphysin 1 is abundant in mature neurons. The graph represents quantification of BIN1 signal intensities normalized to actin. c Enrichment of BIN1:L in the corpus callosum. Adult rat brain was microdissected into different regions and homogenates were analyzed by immunoblotting using BSH3 and cellular marker antibodies. Amph 1 = Amphiphysin 1

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Molecular Neurodegeneration

ISSN: 1750-1326