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Fig. 9 | Molecular Neurodegeneration

Fig. 9

From: Predominant expression of Alzheimer’s disease-associated BIN1 in mature oligodendrocytes and localization to white matter tracts

Fig. 9

Loss of BIN1 parallels myelin loss in multiple sclerosis brain lesions. a Staining of adjacent serial autopsy brain sections with Luxol fast blue (left) and BIN1 antibody (right) shows myelin loss that parallels striking loss of BIN1 labeling intensity within a chronic/inactive multiple sclerosis plaque. b Adjacent sections of samples from multiple sclerosis patients were analyzed by histology or immunohistochemistry. Images correspond to an active lesion (top), shadow plaque (middle) and chronic plaque (bottom). Dashed lines mark the lesion border. c Luxol fast blue (not shown) and BIN1 antibody staining intensities were quantified from digitized images. The percentage difference in the staining intensities within lesions in comparison to adjacent normal white matter was calculated. Correlation matrix analysis reveals a significant correlation between myelin loss and reduction in BIN1 immunolabeling intensities. d Higher magnification of the border of an active lesion depicting myelin fragmentation and loss of BIN1 immunoreactivity. CD68 staining of foamy macrophages (arrows) and GFAP staining of hypertrophic astrocytes (asterisks) are also shown in the bottom panels. NWM = normal white matter

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