Fig. 7

Transgene expression after rAAV injection in the rat substantia nigra and striatum. Adeno-associated viral vectors expressing either PLK2 or mPLK2 were mixed at a 1:1 ratio with a vector encoding for h-asyn and injected into rat substantia nigra or striatum (n = 4 per group). Two weeks after transduction, gene expression was visualized using the asyn211 antibody on sections from ventral midbrain (a–c) or striatum (p–r). Panels (d–f) and (s–u) show vesicular monoamine transporter 2 (VMAT2) stainings for dopaminergic nigral neurons and dopamine- and cAMP-regulated neuronal phosphoprotein 32 (DARPP32) stainings for striatal neurons from the two targeted structures, respectively. Cresyl violet stainings from adjacent sections are shown in (g–i) for the ventral midbrain and in (v–x) for the striatum. High magnification confocal images of transduced cells illustrate co-expression of asyn (green) and PLK2 (magenta) transgenes in nigral (i–o) and striatal neurons (y–d’). Scale bar in (a) and (p) is 0.4 mm and applies to (a–c) and (p–r), in (g) and (v) are 100 μm and apply to (d–i) and (s–x), respectively, and in (m) is 10 μm and applies to (i–o) and (y–d)'