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Table 2 Antibody sensitivity in total (left) and pS129 h-asyn (right) assay is shown as signal to background ratios for each pair

From: A novel multiplex assay for simultaneous quantification of total and S129 phosphorylated human alpha-synuclein

 

AB’s conjugated to Europium Acceptor-beads (50 μg/ml)

LB509

syn211

syn204

4B12

 

EP1536Y

MJF-R13

11A5

Biotinylated AB’s (5nM)

LB509

0.7

N/A

16.4

84.8

 

N/A

N/A

N/A

syn211

N/A

2.5

9.2

233.5

 

N/A

N/A

N/A

syn204

174.1

100.2

1.0

1.1

 

15.3

101.8

21.2

4B12

440.9

576.3

1.1

0.9

 

35.8

114.6

704.2

  1. N/A not analyzed
  2. Bold data signifies most favorable signal to background ratios