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Fig. 10 | Molecular Neurodegeneration

Fig. 10

From: Affinity of Tau antibodies for solubilized pathological Tau species but not their immunogen or insoluble Tau aggregates predicts in vivo and ex vivo efficacy

Fig. 10

Low dose PHF (1 μg/ml) increased the phosphorylated tau/NeuN ratio, which was prevented by 4E6. a, b Immunoblots of samples treated with 1 μg/ml PHF alone or with a 4E6 or b IgG1, reacted with a P-Ser199 antibody. c In cells treated with 1 μg/ml PHF and 4E6, the PHF alone and Ab → PHF groups showed significantly higher phosphorylated tau levels compared to untreated control samples (165 and 185 % above control, p < 0.0001 for both). The PHF + Ab and PHF → Ab groups were significantly lower (91 and 112 % control, p < 0.0001 for both) than the PHF alone samples. d All of the IgG groups showed significantly higher average phospho-tau levels than the untreated controls (79, 119, and 94 % above control, p < 0.0001 for all) after 7 days. None of the groups were significantly different compared to the PHF alone samples. e As above, NeuN levels were used to control for cell loss, and the pattern of results seen in 4E6 treated cells remained. The PHF alone and Ab → PHF groups had significantly higher phospho-tau/NeuN ratios than untreated samples (1.46 and 1.9 fold higher, p < 0.0001). The PHF + Ab and PHF → Ab groups had significantly lower phospho-tau levels than the PHF alone samples comparable to untreated controls. f Correcting for NeuN levels, the same pattern of results in cells treated with control IgG was observed. All groups showed significantly higher levels of phospho-tau/NeuN relative to untreated controls (2, 3.9 and 1.5 fold increase in the PHF + Ab, PHF → Ab, and Ab → PHF groups, p < 0.0001 for all) and no difference relative to the PHF alone samples

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