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Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: Affinity of Tau antibodies for solubilized pathological Tau species but not their immunogen or insoluble Tau aggregates predicts in vivo and ex vivo efficacy

Fig. 5

4E6, but not 6B2, prevented neurotoxicity induced by 10 μg/ml PHF. ac NeuN immunoblots from samples treated with 10 μg/ml PHF alone and with PHF and 1 μg/ml a 4E6, B 6B2 or c control mouse IgG1. d Quantitation of signal in samples treated with PHF alone and a combination of PHF and 4E6. At 10 μg/ml, PHF alone reduced NeuN levels by 94 % relative to untreated control cells (p < 0.0001). PHF + Ab and PHF → Ab paradigms resulted in NeuN levels significantly higher than those incubated with PHF alone (116 and 124 % control, p < 0.0001 for both) and were comparable to control levels. However, the Ab → PHF paradigm was ineffective and showed a significantly reduced NeuN signal relative to control (93 % loss, p < 0.0001) and no improvement over PHF alone samples. e 6B2 did not prevent NeuN loss under any of the dosing conditions used. None of the groups were significantly improved relative to PHF alone. f Similar to the 6B2 samples, the control IgG1 did not protect against the PHF induced loss of NeuN and these samples were similar to the PHF alone group

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