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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Affinity of Tau antibodies for solubilized pathological Tau species but not their immunogen or insoluble Tau aggregates predicts in vivo and ex vivo efficacy

Fig. 6

Low dose PHF (1 μg/ml) reduced NeuN signal, and 4E6 prevented this toxicity. a, b NeuN immunoblots for samples treated with 1 μg/ml PHF alone or 1 μg/ml PHF and 1 μg/ml a 4E6 or b control IgG1. c Chemiluminescent signal was quantified and as in the higher dose samples, exposure to PHF decreased NeuN signal (30 % loss relative to untreated cells, p < 0.001) after 7 days. For 4E6, the Ab → PHF group also showed a decline in NeuN levels (36 % decrease relative to control, p < 0.05) comparable to PHF alone samples. Again the PHF + Ab and PHF → Ab groups had significantly higher NeuN levels at the end of the experiment (102 and 91 % control values, p < 0.01 relative to PHF alone). d Mouse IgG1 was ineffective in preventing NeuN loss under all of the dosing conditions and did not differ significantly from PHF alone

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