Fig. 10

The effect of glutamine on HDAC4 localization. a DIV14 neurons from Atm wildtype (Atm ^+/+), or knockout (Atm ^−/−) were cultured under various dosages of glutamine for 72 h. To access the role of ATM kinase activity, treatment with 2 μM of ATM specific inhibitor (KU-60019) was initiated in DIV9 wildtype neurons for 48 h in complete medium, followed by glutamine treatment at different dosage for another 72 h. b shRNA against Atm was also used to study the effect of losing the protein in mature neurons. shRNA was transfected into DIV9 wildtype neurons for 48 h in complete medium, followed by glutamine treatment at different dosage for another 72 h. Effect on HDAC4 localization was analyzed by immunohistochemistry. Scale Bar = 25 μm. N = 3