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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Deimmunization for gene therapy: host matching of synthetic zinc finger constructs enables long-term mutant Huntingtin repression in mice

Fig. 6

Visualising striatal neuronal density after various treatments. Representative micrographs of Neu-N immunostained striatal coronal slices for the control and injected hemisphere of each treatment at each time point. ZF-KOX1 toxicity is observed in areas of the injected striata that are devoid of marked neurons, 4 and 6 weeks after treatment (a, b), whereas the contralateral hemispheres (a’, b’) show neuronal densities similar to PBS injected (g, h) and untreated hemispheres (g’, h’). Conversely, mZF-KRAB treatment did not significantly affect neuronal density (c, c’, d, d’). Strikingly, GFP injections did not affect neuronal density at 4 weeks after treatment (e, e’), but caused a delayed strong toxic response that reduced neuronal density both in the injected (f) and the contralateral hemisphere (f’), 6 weeks post-injection. Scale bar: 100 μm

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