|
Treatment
|
Weeks post-injection
|
Linear regression
|
Htt (4,7) %
|
Linear regression
|
Atn1 (3,10) %
|
Linear regression
|
Atxn2 (6,10) %
|
Linear regression
|
Tbp (3,13) %
|
|---|
|
ZF-KOX1
|
2
|
R
2
= 0.97
§
|
92.1 ± 0.4*
|
R
2 = 0.03
|
101.2 ± 13.8
|
R
2 = 0.09
|
95.5 ± 3.9
|
R
2
= 0.98
§
|
94.5 ± 5.0
|
|
4
|
R
2
= 0.99
§
|
89.6 ± 2.4*
|
R
2 = 0.64
|
91.7 ± 13.7
|
R
2 = 0.57
|
96.1 ± 6.8
|
R
2 = 0.98
|
91.5 ± 1.7
|
|
6
|
R
2
= 0.99*
|
90.6 ± 9.6
|
R
2 = 0.09
|
106.3 ± 4.9
|
R
2 = 0.96
|
98.4 ± 2.9
|
R
2 = 0.65
|
98.1 ± 3.3
|
|
mZF-KRAB
|
2
|
R
2 = 0.53
|
98.3 ± 3.3
|
R
2 = 0.02
|
109.4 ± 10.6
|
R
2 = 0.00
|
103.3 ± 11.8
|
R
2 = 0.08
|
99.1 ± 7.9
|
|
4
|
R
2 = 0.14
|
88.4 ± 6.7
|
R
2 = 0.19
|
88.6 ± 7.3
|
R
2 = 0.36
|
89.6 ± 5.4
|
R
2 = 0.43
|
90.5 ± 10.4
|
|
6
|
R
2 = 0.43
|
92.5 ± 11.35
|
R
2 = 0.16
|
91.4 ± 6.5
|
R
2 = 0.00
|
96.7 ± 4.4
|
R
2 = 0.07
|
94.7 ± 4.8
|
- The first number (in brackets after the name of the gene) represents the number of CAG repeats, the second the number of glutamines in the coding stretch (CAG + CAA). Values are given as the percentage expression of the gene of interest, with respect to the average values in the control hemispheres. The result of ZF-KOX1, 6 weeks post-injection, should be taken with caution, since at this time point there was a significant neuronal loss and leakage of the vector to the contralateral hemisphere. In bold: §
P < 0.1; *P < 0.05. Atn1: atrophin 1; Atxn2: ataxin 2; Htt: huntingtin (mouse); Tbp: TATA binding protein
