Fig. 11
Expression of NHE6∆ES decreases the complexity of dendritic arborisation in primary hippocampal neurons. a Confocal images of mouse primary hippocampal neurons transfected with cytosolic GFP alone or co-transfected with GFP and monomeric cherry fluorescent protein-tagged NHE6 (NHE6ChFP) WT or ∆ES. Forty-eight h post-transfection, cells were fixed in 4 % paraformaldehyde, mounted onto glass slides, and examined by confocal microscopy. Images show each channel individually. b-d Quantification of parameters related to neuronal branching, including the sums of total dendritic length (b) and area (c), as well as the total number of branch points per cell (d), using the FilamentTracer plug-in module from Imaris Software. Transfection of NHE6ChFP-∆ES appeared to reduce the extent of neuronal arborisation, as can be discerned from the representative images in a. Data from four experiments is represented as mean ± S.E.M. values. *: p < 0.01, one-way ANOVA with Tukey post-hoc test. Scale bar: 60 μm