Skip to main content
Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: A Christianson syndrome-linked deletion mutation (∆287ES288) in SLC9A6 disrupts recycling endosomal function and elicits neurodegeneration and cell death

Fig. 2

Biosynthetic maturation of NHE6 is reduced for the ∆ES mutant. AP-1 cells were transiently transfected with a NHE6v1HA WT or b ΔES and lysed at the indicated time points over a 48 h period. Equal amounts of proteins were subjected to SDS-PAGE and immunoblotting with a monoclonal anti-HA antibody (α-HA). The identities of the various NHE6 bands are as described in the legend to Fig. 1. For the ΔES immunoblot in panel B, a longer X-ray film exposure (18X) of the 36 h and 48 h time points is also shown. The same immunoblots were also probed with a monoclonal anti-β-tubulin antibody as a loading control. c-d Densitometric quantification of the relative abundances of the monomeric and dimeric forms of WT or ΔES was assessed using ImageJ software and expressed as ratios of fully glycosylated/total protein (fg/total). For quantification, multiple exposures of the immunoblots were taken to ensure the signal intensities of the bands were within the linear range of the X-ray film. Data are shown as mean ± standard error of the mean (S.E.M.) of four different experiments

Back to article page