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Table 3 CSF collection procedures and sample processing

From: No diurnal variation of classical and candidate biomarkers of Alzheimer’s disease in CSF

Subjects: 13 neurosurgical patients carrying a CSF drainage
8 M, 5 F; 26 to 82 y
Admitted for tumor, trauma, hemorrhage (n = 9), CSF pressure monitoring (n = 4)
CSF collection: From lumbar (n = 7) or ventricular (n = 6) drainage
Volume: 4 mL in PP Sarsted tubes, code: 62.610.201
Time of day: 8.00, 12.00, 16.00, 20.00, 00.00, 8.00
CSF processing: Centrifugation within 15 min, 2000 g × 10 min at RT
0.5 mL aliquots in PP Sarsted tubes, code: 72.730.007
CSF storage: −80 °C freezer, with controlled temperature, alarm and CO2 backup system
Thawing: RT with gentle shaking
Biomarker measurement: Immunoassay (Fujirebio): Aβ1-42, t-tau, p-tau181
Immunoassay (Biovendor): VILIP-1
Immunoassay (R&D): YKL-40
Immunoassay (Euroimmun): Aβ1-38, Aβ1-40, Aβ1-42, t-tau, ApoE
Immunoassay (in-house): neurogranin
Immunoassay (MSD): Aβx-38, Aβx-40, Aβx-42, sAPPα, sAPPβ