Fig. 4
From: Progranulin promotes peripheral nerve regeneration and reinnervation: role of notch signaling
Immunofluorescent analysis of long-distance axonal regrowth after crush injury of the sciatic nerve. a Time course of rainbow pseudo-color images of EYFP immunofluorescent intensities along the injured sciatic nerve in SLICK-Grn mice (without tamoxifen) and SLICK-Grn-OE mice (with tamoxifen). Tiled images were used for reconstruction of the nerves. The 2D line intensity plots show EYFP intensity of the ipsilateral (filled area) and the contralateral nerve (line), which was used as control (procedure of quantification in c). Scale bars 500 μm. b PKG1 immunofluorescent intensities and quantification in analogy to a. c Illustration of the procedure of intensity quantification along the nerve. Line intensity profile plots through the middle of the nerve were obtained in ImageJ after background subtraction and automatic setting of the intensity threshold, then exported and smoothed employing the Loess function. d Time courses of the areas under the smoothed intensity profile plots (AUCs), calculated according to the linear trapezoidal rule (means ± SD; * P < 0.05, rm-ANOVA with subsequent t-tests employing a Bonferroni correction of alpha)