Fig. 7

Up-regulation of Fgfr4 in astrocytes after SCI. Bar graph displaying increased Fgfr4 transcript expression in astrocytes (a). Confocal micrographs confirming astrocytic Fgfr4 protein over-expression after injury in Aldh1l1-EGFP mice (l–u) as compared to un-injured mice (b–k). Morphologically, Fgfr4 expression was identical to GFAP and eGFP profiles in Aldh1l1-EGFP mice. DAPI staining (b, g, l & q). Bar graphs displaying increased Fgfr4 protein expression at multiple stages after FT injury (v–x). Note that injury-induced increase in Fgfr4 protein expression is shown as an average of both rostral and caudal segments combined (green rectangles, v) as well as rostral (w) and caudal (x) segments individually. Scale bars (b–f): 50 μm, (g–k): 20 μm, (l–p): 25 μm, (q–u): 10 μm. a Values are actual fold change ± SEM (*p < 0.05; **p < 0.01 by t test). v–x Values are area fractions ± SEM (*p < 0.05; **p < 0.01; ***p < 0.001 by un-paired t test). b–k un-injured and (l–u) FT 4 weeks