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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Seizure protein 6 and its homolog seizure 6-like protein are physiological substrates of BACE1 in neurons

Fig. 1

Specificity of SEZ6 and SEZ6L monoclonal antibodies. a Membranes from mouse brains were probed with the indicated antibodies against SEZ6, SEZ6L, SEZ6L2 or calnexin. Brains were collected from wild type (WT), SEZ6-/- (SEZ6 KO), SEZ6L2-/- (SEZ6L2 KO) or triple knock-out (TKO) mice lacking SEZ6, SEZ6L and SEZ6L2. b Lysates from primary neurons were treated with peptide N-glycosidase F (PNGaseF) or endoglycosidase H (EndoH) and blotted for SEZ6 and SEZ6L. For SEZ6, a polyclonal antibody was used in the deglycosylation experiment. * indicates mature SEZ6, ** indicates immature SEZ6. c, d Immunohistochemistry of TKO and WT brains using antibody against SEZ6 (c) or SEZ6L (d)

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