Skip to main content
Fig. 7 | Molecular Neurodegeneration

Fig. 7

From: Glymphatic distribution of CSF-derived apoE into brain is isoform specific and suppressed during sleep deprivation

Fig. 7

ApoE3 levels in brain cells increase following lenti-apoE3 transduction of the choroid plexus. a Lenti-apoE3 effectively transduced the choroid plexus and ependymal layer at 1 week post-transduction. ApoE3 immunolabeled with a human specific anti-apoE antibody. b-d Choroid plexus expression of apoE3 at 8 weeks post-transduction. Scale bar 100 μm. AQP1 (red; b); apoE3 (green; c) and merged images (d). e-j Parenchymal human apoE3 (green) at 1 (e-f), 4(g-h) and 8 (i-j) weeks post-transduction. Scale bar 100 μm. Neurons: NeuN-positive cells (blue). Astrocytes: GFAP -positive cells (red). k Human specific anti-apoE does not react with endogenous mouse apoE. l Quantification of apoE3 intensities in neurons (apoE co-localization with NeuN-positive cells) with post-transduction time. Lenti-apoE3 delivered intraventricularly (3 μL lenti- apoE (4.06 × 108 TU/ml) and after 1 to 8 weeks the brains were perfusion fixed (PFA) followed by immunolabeling of brain sections. Representative images from 5 mice per group. Values are mean ± SEM. N = 5

Back to article page