Fig. 8

Increased CSF and brain apoE3 levels in lenti-apoE3 transduced choroid plexus. a Representative Western blots of apoE3 standards (top) and apoE3 in CSF and plasma from lenti-EGFP (controls) and lenti-apoE3 transduced mice. Quantification of CSF apoE3 levels by Western blot analysis at 4 weeks post-transduction (b), and by ELISA at 4 (left) and 8 (right) weeks post-transduction (c). d Levels of brain endogenous apoE and human apoE3 in the transduced mice. e Quantification of mouse apoE levels by Western blot analysis. Values are mean ± SEM. N = 4-5. Intraventricular (unilateral) injection of 3 μL lenti-human apoE (4.06 × 10⁸ TU/ml) or 3 μL lenti-EGFP (10¹² TU/ml). At 4 and 8 weeks post-transduction samples of CSF, plasma and brain were collected