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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: 3D culture models of Alzheimer’s disease: a road map to a “cure-in-a-dish”

Fig. 1

Recapitulation of Aβ and Tau pathology in a 3D human neural cell culture model of AD. Human neural progenitor cells (hNPCs) are virally transfected with APP and/or PSEN1 FAD mutations with either GFP or mCherry as a reporter for viral infection. These cells are enriched based on GFP and/or mCherry signals by FACS, and then differentiated in 3D Matrigel culture systems. Thin-layer (~100–300 μm) culture format is suited for immunostaining analyses and thick-layer (~4 mm) culture format is used for biochemical analyses. In 3D-differentiated hNPCs with FAD mutations, extracellular Aβ aggregates develop ~6 weeks of differentiation and robust increase in tauopathy is evident from ~10–14 weeks of differentiation

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