Fig. 5

Argyrophilic inclusion pathology is attenuated in IL-6 expressing mice injected in the hippocampus with αSyn fibrils. Neonatal wild type mice were injected with rAAV-EV or rAAV-IL-6 and subsequently bilaterally injected in the hippocampus with αSyn fibrils at 2 months of age. Mice were analyzed 4 months post injection with αSyn fibrils in the hippocampus. Representative images of hippocampal and cortical regions of mice stained with modified Campbell-Switzer silver staining show that mice overexpressing IL-6 have fewer silver-positive inclusions than control (EV) mice (a). Red dots show the comparative distribution of argyrophilic inclusions along rostral-caudal axis as identified by modified Campbell-Switzer silver staining (b). n = 4–5 mice/cohort. Scale bar, 150 μm (left panel) and for middle and right panels, 30 μm and 15 μm (inset)