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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: The release and trans-synaptic transmission of Tau via exosomes

Fig. 6

Uptake of TauGFP exosomes by neurons and microglia in cultured organotypic hippocampal slices. a Immunofluorescence assay showing the uptake of TauGFP exosomes by hippocampal slice cultures. Hippocampal slice cultures were incubated for 24 h with exosomes (20 μg) isolated from N2a cells expressing TauGFP. Cultures were afterwards stained for neuronal marker MAP2 (red, a1), microglial marker Iba1 (red, a2, a3) or astrocytic marker GFAP (red, a4). TauGFP colocalizes with MAP2 (A1) and Iba1 (a2, a3) suggesting the uptake of exosomes by neurons (a1) and microglia (a2, a3). Nearly no colocalization of TauGFP with GFAP is observed (a4), indicating no internalization of exosomes by astrocytes. Scale bars = 10 μm. b Quantification of the uptake of TauGFP exosomes by neurons, microglia and astrocytes shown in (a). Significantly higher percentage of microglia (24.8 ± 14.1%) than neurons (12.3 ± 11.2%) exhibits uptake of exosomes (n = 22–24 slices). Nearly no uptake of exosomes is observed in astrocytes. Error bars: SEM. ANOVA: *** p < 0.001

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