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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Tdp-43 cryptic exons are highly variable between cell types

Fig. 1

Generation of CaMKIIα-Cre;Tardbp F/F and MLC-Cre;Tardbp F/F knockout mice. (a) Breeding strategy to cross floxed Tardbp knockout mice with CaMKIIα-Cre or MLC-Cre mouse lines to conditionally delete Tdp-43 in excitatory neuron or skeletal muscle, respectively. Hippocampal protein extracts from CaMKIIα-Cre;Tardbp F/F knockout mice were taken from p25 and 3-month old mice, as indicated. Protein extracts from various muscle groups, as indicated, were taken from 2-month old MLC-Cre;Tardbp F/F mice. Immunoblotting confirms deletion of Tdp-43 in the hippocampi of CaMKIIα-Cre;Tardbp F/F knockout mice (b) and the quadriceps of MLC-Cre;Tardbp F/F knockout mice (c); biological replicates of immunoblotting were performed in excess of n = 3 to validate knockdown. (d) Immunofluorescence staining of hippocampal sections from 3 month old CaMKIIα-Cre;Tardbp F/F knockout mice demonstrate specific deletion of Tdp-43 from neurons (CA region, scale bar = 50 μm). (e) Immunohistochemical staining of Tdp-43 in quadriceps from 3 month old MLC-Cre;Tardbp F/F knockout mice also reveals loss of Tdp-43, as indicated by asterisks (scale bar = 50 μm)

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