Fig. 3

Immunohistochemical examination of human SOD1-ALS cases (C111Y mutation) with anti-SOD1^olig antibody. DAB staining of a a sacral spinal cord (ventral horn) section of the case IV-6 and b a cervical spinal cord (ventral horn) of the case III-5 was performed using anti-SOD1^olig antibody. c-f Serial sections of a lumbar spinal cord (ventral horn) of the case IV-6 were immunostained with c, e anti-SOD1^olig and d, f anti-SOD1 (clone 1G2, MBL) antibodies. Sections shown in c and d or e and f are serial. Nuclei were also stained by hematoxylin (blue). The bars represent 50 μm