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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Immunochemical characterization on pathological oligomers of mutant Cu/Zn-superoxide dismutase in amyotrophic lateral sclerosis

Fig. 4

Anti-SOD1olig antibody reacts with the interior of SOD1 structure. a The translated products of five exons in SOD1 and the three peptides, Pep 1, 2, and 3, are shown in schematic representation of the SOD1 primary structure. The ligands for copper and zinc ions are also shown and colored blue and red, respectively. His 63 (colored green) is a ligand for bridging both copper and zinc ions. b Identification of the epitope for anti-SOD1olig antibody (filled bars) was performed by indirect ELISA using the dissected peptides of SOD1 shown in (a). The peptides were prepared as a fusion protein with an N-terminal 6x His tagged GST. Almost equal amounts of peptides were examined, which was confirmed by ELISA using anti-His tag antibody (sc-8036, Santa Cruz Biotechnology) (open bars). The ELISA signal was represented as a ratio against that obtained using BSA. c The region covering the epitope of anti-SOD1olig antibody (Gly44 - Asn53) is shown red in the crystal structure of SOD1 (PDB ID: 2C9V). Copper (blue) and zinc (orange) ions are also indicated with the ligands

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