Fig. 4From: GDE2 is essential for neuronal survival in the postnatal mammalian spinal cord Gde2 KO neurons show cytoskeletal dysregulation. a–l Neurofilament-H immunostaining in the ventral horn. Arrows indicate cytoskeletal accumulation in the absence of Gde2. Arrowheads mark spheroid deposits of Neurofilament-H within dilated neuronal processes. Scale bar a, b, g, h = 25 μm. Scale bar c-f, i-l = 15 μm. m Western blot quantifying increased NF-H protein. Graph represents mean ± SEM, *p = 0.004, Student’s t test, n = 3 WT, 4 KO. n–q Gde2 null neurons accrue peripherin protein. Arrows indicate peripherin tendrils in the cell body at 6 weeks and axons at 19 months. Scale bar = 25 μm. r–s” Arrowheads denote axonal accumulation of peripherin enriched around the perimeter of the axon. Scale bar = 10 μm. n = 3Back to article page