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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Pathogenic LRRK2 variants are gain-of-function mutations that enhance LRRK2-mediated repression of β-catenin signaling

Fig. 3

Lrrk2 knockout cells display increased Axin2 promoter activity. Wild-type and Lrrk2 MEFs were co-transfected with a reporter plasmid containing the murine Axin2 promoter upstream of luciferase and TK-renilla. The relative luciferase activity of resultant cell lysates was measured 24 h post-transfection. a In the absence of canonical Wnt pathway activation, Axin2 promoter activity was significantly higher in Lrrk2 knock-out cells than wild-type controls (n = 18; T-test, p < 0.001). b Co-transfection with FLAG-β-catenin or empty vector revealed a marked activation of the Axin2 reporter by β-catenin in Lrrk2 knockout cells (n = 9; 1-way ANOVA (F = 8.037, p < 0.001) followed by Bonferroni post-hoc analysis, p < 0.01 versus all other conditions)

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Molecular Neurodegeneration

ISSN: 1750-1326