Fig. 10

Adult-only lead exposure results in elevated blood lead levels and lead deposition in the brain. 8-week-old ApoE3-KI and ApoE4-KI male and female mice were exposed to 0.2% lead acetate for 12 weeks and then sacrificed. Blood and one brain hemisphere were collected at sacrifice and (a) blood lead and (b) brain lead levels were measured using ICP-MS. Brightfield images of one brain hemisphere from a female (c) control and (d) lead-treated ApoE4-KI mouse after the 12 week lead exposure. Semi-quantitative measurement of lead in the brain of a (e) control and (f) lead-treated ApoE4-KI mouse using LA-ICP-MS. Two-way ANOVA with Fisher’s LSD post-test: n.s., not significant; ** p < 0.01; *** p < 0.001. Scale bars, 100 μm