Fig. 11

Adult lead exposure decreases adult-born cell proliferation in the DG of the hippocampus of all mice. 8-week-old male and female ApoE3- and ApoE4-KI mice were exposed to 0.2% lead acetate for 12 weeks and then sacrificed. BrdU was administered 2 h prior to sacrifice (1 x 100 mg/kg). Representative images of BrdU (red) immunostaining in the DG of 20-week-old (a) female and (b) male ApoE3-KI and ApoE4-KI control and lead-treated mice. Quantification of the total BrdU+ cells per DG in (c) females and (d) males. Quantification of the percent change in total BrdU+ cells in lead-treated mice relative to ApoE3-KI or ApoE4-KI control (e) females and (f) males. Data are mean ± SEM with n = 3–5 per genotype/sex/treatment. Two-way ANOVA with Fisher’s LSD post-test: n.s., not significant; * p < 0.05; ** p < 0.01. Scale bars, 100 μm