Fig. 12

Lead decreases adult-born immature neuron maturation in the DG of ApoE4-KI female mice. 8-week-old male and female ApoE3- and ApoE4-KI mice were exposed to 0.2% lead acetate for 12 weeks and then sacrificed. 100 mg/kg BrdU was administered 5 times in 1 day (every 2 h) 3 weeks prior to sacrifice (i.e., at 9 weeks into lead exposure). a Representative images of BrdU (red) and DCX (green) immunostaining in the DG of control and lead-treated female ApoE3-KI and ApoE4-KI animals. Quantification of the percent of total BrdU⁺ cells that are BrdU⁺DCX⁺ per DG in females (b) and males (c) respectively. Data are mean ± SEM with n = 4–5 per genotype/sex/treatment. Two-way ANOVA with Fisher’s LSD post-test: Comparison between control and lead-treated: n.s., not significant; * p < 0.05; *** p < 0.001. Comparison between males and females of the same genotype and treatment: n.s., not significant; ##, p < 0.01. Scale bar, 50 μm