Fig. 2

Lead-treated ApoE3-KI and ApoE4-KI male and female mice do not exhibit locomotor deficits in the open field test compared to controls. Mice were exposed to lead as previously described and the open field test was conducted after the cessation of the lead exposure. There was a significant main effect of lead treatment in female animals on floor plane distance, moving time, and average speed (Two-way ANOVA: floor plane distance, F(1,31) = 22.85, p < 0.0001; moving time, F(1,31) = 20.47, p < 0.0001; average speed, F(1,31) = 20.49, p < 0.0001). Post-hoc analyses found that lead-treated ApoE3-KI and/or ApoE4-KI females traveled a (a) greater distance, (b) spent the same or more time moving, and (c) traveled slightly faster than controls. There were no significant differences between lead and control ApoE3-KI and ApoE4-KI males or between lead-treated ApoE3-KI and ApoE4-KI mice (males or females) in any of the locomotor endpoints. Data are mean ± SEM with n = 8–13 per genotype/sex/treatment. Two-way ANOVA with Holm-Sidak post-hoc tests: n.s., not significant; * p < 0.05; *** p < 0.001