Fig. 2

FK506 attenuated the decrease of TH immunoreactivity in the striatum and substantia nigra of MPTP injected humanized CD34+ mice. The hCD34+ mice female mice were intraperitoneal injected three times with saline vehicle or MPTP-HCL (18 mg/kg) at 2 h intervals followed by FK506 injections (10 mg/kg/day) for 5 days. Eight days post MPTP injections, brains were dissected out and right hemispheres fixed and immunostained using anti-TH antibody. a Representative images from striatum and substantia nigra are shown at 1× magnification. b Optical density of immunopositive staining from striatum and c number of TH-positive cells from substantia nigra were measured and averaged 4-5 sections per brain and 4–5 animals per group ± SD (*p < 0.05)