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Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: Defining the contribution of neuroinflammation to Parkinson’s disease in humanized immune system mice

Fig. 5

MPTP injections increased microgliosis in the striatum, substantia nigra, and hippocampus of C57BL/6 mice. C57BL/6 female mice were intraperitoneal injected three times with saline vehicle or MPTP-HCL (18 mg/kg) at 2 h intervals followed by FK506 injections (10 mg/kg/day) for 5 days. Eight days post MPTP injections, brains were dissected out and right hemispheres fixed and immunostained using anti-Iba-1 antibody (microglia marker). a Representative images from striatum, substantia nigra and hippocampus are shown at 10× magnification with 63× magnification insert. Optical density of immunopositive staining from b striatum and d hippocampus were measured for 3–4 optical fields at 10× magnification per section from 4 to 5 animals per group and c number of Iba-1 positive cells from substantia nigra were counted and averaged ± SD (*p < 0.05)

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