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Fig. 7 | Molecular Neurodegeneration

Fig. 7

From: Dynamic presenilin 1 and synaptotagmin 1 interaction modulates exocytosis and amyloid β production

Fig. 7

Inhibition of PS1-Syt1 binding leads to the alterations in synaptic vesicle morphology and distribution. Representative electron micrographs show synaptic vesicles within the synapses of the primary neurons pre-treated for 2 h with scramble or PS1-LNT peptides, and stimulated for 15 min with 50 mM KCl. Enlarged synaptic vesicles in the PS1-LNT treated synapses are shown by red asterisks. Scale bar 100 nm. The graphs show the quantification of: a synaptic vesicle size; b density; and c distance from the active zone. The data are normalized to scramble-treated cells and presented as mean ± SEM, n = 100, n = total number of synapses analyzed in three independent experiments. Graph (d) presents the frequency distribution of the synaptic vesicles within individual synapses as a function of the distance from the active zone. Statistical significance was determined using two-tailed unpaired Student’s t-test (a, b, c) or two-way ANOVA followed by Bonferroni’s post-test (d), *p < 0.05, **p < 0.01, ***p < 0.001

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