Fig. 4

tPA promotes oligodendrocyte migration along vessels during development at times of intense migration. a Photomicrographs from spinal sections show representative images (from 3 mice) of tPA (red), CD31 (green) and Olig2 (grey) immunostaining in the right ventral horn of WT mice spinal cords at E13. Right image is a 3D reconstruction from confocal acquisitions (on the left). b Photomicrographs from tissue sections show representative images (from 3 mice per group) of Olig2 (red), CD31 (green), and DAPI (blue) immunostaining in the right ventral horn of WT and tPA^−/− mice spinal cords at E13. c Quantification of the percentage of Olig2⁺ oligodendrocytes close (<10 μm) to vessels in the ventral spinal cord parenchyma outside the pMN domain in WT and tPA^−/− mice (mean ± SEM, n = 3 per group; *p < 0.05). d Quantification of the percentage of CD31⁺ staining area in the ventral spinal cord parenchyma outside the pMN domain in WT and tPA^−/− mice (mean ± SEM, n = 3 per group; *p < 0.05). E: embryonic day; OL: oligodendrocyte; pMN domain: motor neuron progenitor domain; WT: wild type. Scale bars: 20 μm