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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Spatiotemporal progression of ubiquitin-proteasome system inhibition after status epilepticus suggests protective adaptation against hippocampal injury

Fig. 2

Subfield-specific UPS impairment after status epilepticus in the hippocampus. a. Representative Western (n = 1 per lane) and graphs showing increased GFP protein in the different ipsilateral hippocampal subfields DG, CA1 and CA3 at different time-points following status epilepticus in UbG76V-GFP mice (mean ± sem, *p <0.05 by one-way ANOVA with Fisher’s post hoc test; n = 4 (0 h), 5 (24 h) and 6 (Con, 1 h, 4 h and 8 h)). b. Representative Western (n = 1 per lane) showing increased polyubiquitination (FK2 antibody) in C57/Bl6 wild-type mice after status epilepticus which is most prominent in the DG and CA1 subfield. c. Graph showing decreased chymotrypsin-like proteasome activity in the CA1 subfield of C57/Bl6 wild-type mice 4 h after status epilepticus when compared to control-injected mice. No changes could be observed in the remaining subfields DG (p = 0.289) and CA3 (p = 0.291) at the same time-point post-status epilepticus (4 h) (mean ± sem, **p <0.01 by student’s two-tailed t-test; n = 8 (Con, Control) and 6 (SE, status epilepticus) per group). d. Graphs showing no significant changes in GFP mRNA levels 4 h and 8 h after status epilepticus for the ipsi-lateral hippocampal subfields DG, CA1 and CA3 (mean ± sem, by one-way ANOVA with Fisher’s post hoc test; n = 4 per group). ns = non-significant

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