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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Spatiotemporal progression of ubiquitin-proteasome system inhibition after status epilepticus suggests protective adaptation against hippocampal injury

Fig. 4

Cell-specific UPS impairment after status epilepticus. a. Representative photomicrographs (5x lens) of coronal cut hippocampal brain slices from UbG76V-GFP reporter mice double stained against GFP (green) and the neuronal marker NeuN (red) at different time-points post-status epilepticus. Scale bar, 500 μm. b. Representative photomicrographs (40x lens) showing co-localization between the neuronal marker NeuN (red) and GFP (green) in the hippocampal subfields DG, CA1 and CA3 4 h post-status epilepticus in UbG76V-GFP reporter mice. Scale bar, 50 μm. c. Representative photomicrographs (40x lens) showing co-localization between the astrocyte marker GFAP (red) and GFP (green) in the ipsilateral hippocampal subfields DG, CA1 and CA3 24 h and 72 h post-status epilepticus in UbG76V-GFP reporter mice. Scale bar, 50 μm. d. Graph showing percentage of hippocampal astrocytes positive for GFP after the induction of status epilepticus (mean ± sem, **p <0.01 by one-way ANOVA with Fisher’s post hoc test; n = 4 per group). e. Representative photomicrograph (40 x lens) showing co-localization between GFP (green) and the inter-neuronal marker parvalbumin (PV) (red) in the hilar sub-region of the ipsilateral hippocampus of UbG76V-GFP reporter mice 24 h post-status epilepticus. Scale bar, 50 μm. f. Representative photomicrograph (40 x lens) showing co-localization between GFP (green) and the microglial marker CD11b (red) in the CA3 ipsilateral hippocampal subfield of UbG76V-GFP reporter mice 24 h post-status epilepticus. Scale bar, 50 μm

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