Fig. 2

HDAC6 inhibition decreases Aβ-induced ROS and Ca²⁺. HT22 cells were treated with TBA (0.5 μM, 3 h) after being treated with Aβ (2 μM, 24 h). a ROS level was measured by DCFDA assay in HT22 cells. Upper panel shows representative DCFDA signals (top row) and bright field images (bottom row). Lower panel shows quantitative graph of fluorescent intensity (n = 5, independent experiments). b Ca²⁺ level was measured by Fluo-4 assay in HT22 cells. Left panel is representative images and right panel is quantification of fluorescent intensity (n = 5, independent experiments). Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 (two-way ANOVA, Bonferroni post-hoc test). Scale bar: 100 μm