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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Tetraspanin 6: a pivotal protein of the multiple vesicular body determining exosome release and lysosomal degradation of amyloid precursor protein fragments

Fig. 2

Subcellular localization of TSPAN6 in HEK293 cells. HEK293 cells were transfected with myc-tagged TSPAN6 (myc-TSPAN6), fixed and immunostained for EEA1 as an early-endosomes marker (upper panels), LAMP1 and CD63 as markers for late-compartments (middle panels), and GM130 to visualize the Golgi organelle (lower panels). Myc-TSPAN6 (detected with a rat monoclonal anti-myc antibody) shows a high degree of co-localization (arrows) with late-compartments (detected with a mouse monoclonal antibody against LAMP1 and a rabbit polyclonal antibody against CD63), and a lower co-localization with early endosomes (detected with a mouse monoclonal antibody against EEA1). Low co-localization was observed with the Golgi organelle (detected with a monoclonal antibody against GM130). Scale bars = 10 μm

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