Fig. 5

Acute NWL-117 administration reverses novel object recognition deficits in Casp6-overexpressing KI/Cre mice. a Experimental design for the in vivo study highlighting the novel object recognition (NOR) tests and injections. b Percent touches of objects during the NOR task in WT/WT and WT/Cre controls (n = 16), and Casp6-expressing KI/Cre (n = 9) mice prior to injections. Statistical analysis was performed by one-way ANOVA (p < 0.0001). c Distance traveled, (d) percent time moving, and (e) number of cell entries of control (n = 16) and KI/Cre (n = 9) mice. Statistical analysis was performed by unpaired two-tailed t test. No significant differences were found in C-E. f Percent touches of objects during the NOR task following saline (n = 8) or 20 mg/Kg NWL-117 (n = 8) injections in control mice. Statistical analysis was performed by one-way ANOVA (p < 0.0001). g Percent touches of objects during the NOR task in pre- and post-injections (n = 4) saline injections in KI/Cre mice. Statistical analysis was performed by repeated measures ANOVA (p = 0.7661). h Percent touches of objects during the NOR task in pre- and post- (n = 5) 20 mg/Kg NWL-117 injections in KI/Cre mice. Statistical analysis was performed by repeated measures ANOVA (p = 0.0860) with Bonferroni’s multiple comparison test (* p < 0.05). i Distance traveled, (j) percent time moving, and (k) number of cell entries of control mice injected with saline (n = 8) or NWL-117 (n = 8) and KI/Cre injected with saline (n = 4) or NWL-117 (n = 5). Statistical analysis was performed by two-way ANOVA for panels (i) to (k) and no significant differences were found. For panels (a–k), data represent the mean ± SEM and post hoc analyzes were performed using Bonferroni’s multiple comparison test (*p < 0.05, **p < 0.01, and ***p < 0.001) unless stated otherwise