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Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: Decreased cortical FADD protein is associated with clinical dementia and cognitive decline in an elderly community sample

Fig. 5

Immunofluorecence characterization of FADD in the DLPFC of neuropathology-free NCI (n = 3) and Alzheimer’s diseased (AD; n = 3; two different subjects shown) MAP participants. Single-channel (in greys) or merged (in RGB) confocal images correspond to triple co-immunolabeled sections with antibodies against FADD (H181, Santa-Cruz, 1:50) combined with either (a) synatxin-1 (STX1, clone SP7, locally produced, 1:1000) and NeuN (Chemicon, clone A60, 1:250), or (b) beta-amyloid (Aβ, clone 6 F/3D, Dako, 1:100) and misfolded, pathologic tau (clone Alz-50, locally produced, 1:500). In merged images, colors were arbitrarily assigned (as indicated at the top) to maximize overlap visualization. Overlap panels on the right are ImageJ-generated bitmaps highlighting those pixels where significant colocalization over an unbiased threshold of intensities between the indicated channels were detected in the corresponding pairwise colocalization analyses. Note the change in FADD localization in NCI (mainly in neuronal nuclei and soma, and also some neuropil staining) compared to AD (redistributed to dystrophic neurites, tangles, and within amyloid plaques) brains. Scale bars: 30 μm

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