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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Soluble oligomeric amyloid-β induces calcium dyshomeostasis that precedes synapse loss in the living mouse brain

Fig. 2

Oligomeric Aβ induces calcium overload in neuronal cell bodies and dendritic spines. Neuronal cell bodies (a,b) or dendritic spines (c,d) were imaged before and 1 h after treatment with conditioned media, and the YFP/CFP ratio and resting calcium were calculated for each compartment. Images were pseudocolored according to calcium concentrations. b In cell bodies, TgCM, but not WtCM, induced an increase in resting calcium. (TgCM: n = 165 cells, WtCM: n = 157 cells, in 5 mice, pair-wise comparison of the relative change in YC ratio, * p = 0.0458). d TgCM effect on YC ratio in dendritic spines followed the same trend as neurites and was significantly different from that of WtCM and depleted-TgCM (pair-wise analysis TgCM vs. WtCM * p = 0.0466; TgCM vs. depleted-TgCM * p = 0.0494; WtCM vs. depleted-TgCM p = 0.9916). Scale bars = 20 μm in (a) and 5 μm in (c)

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Molecular Neurodegeneration

ISSN: 1750-1326