Fig. 3

Oligomeric Aβ decreases spine density at 24 h. a Representative images of YFP-filled neurites and dendritic spines before and after 1 h or 24 h treatment with WtCM, TgCM and TgCM + MK-801 (Scale bar = 10 μm). b Box plots showing the relative changes in percentage of spine densities after treatment accordingly to the mean spine density calculated for each animal before treatment. Whiskers represent the minimal and maximal values of each experimental group (n = 3–4 mice per group. For WtCM: n = 58 neurites and 578 spines evaluated after 1 h and n = 91 neurites and 1382 spines evaluated after 24 h. For TgCM: n = 68 neurites and 760 spines evaluated after 1 h and n = 69 neurites and 635 spines evaluated after 24 h. For TgCM + Mk-801: n = 152 neurites and 854 spines evaluated after 1 h and n = 174 neurites and 1044 spines evaluated after 24 h. Linear mixed effects model was fitted with treatment group and time of imaging (as outlined in the graph) as fixed effect and mouse as random effect. (pair-wise comparisons: TgCM 1 h vs. 24 h: *** p < 0.0001, Wtcm 1 h vs. 24 h p = 0.3897, TgCM + MK-801 1 h vs. 24 h p = 0.6667)