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Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: Soluble oligomeric amyloid-β induces calcium dyshomeostasis that precedes synapse loss in the living mouse brain

Fig. 5

Oligomeric Aβ induces calcium overload through activation of NMDA receptors in vivo. The antagonists MK-801 and Dantrolene were applied 10-min before and during TgCM application. Distribution curves of [Ca2+] i (YFP/CFP ratio on the lower x-axis and [Ca2+] i on the upper x-axis) are presented before (purple curve) and after treatment (red curve) for mice treated with WtCM (a) TgCM (b) MK-801 + TgCM (c), dantrolene + TgCM (d) and MK-801 alone (e). The black line indicates the threshold for calcium overload. The percent of overloaded neurites is noted on each graph. Treatment with MK-801, but not of dantrolene, abolished the effect of TgCM on resting calcium (pair-wise comparisons of the relative change in YC ratio: TgCM vs. TgCM + MK-801 p = 0.0221; TgCM vs. TgCM + Dantrolene p = 0.7656; TgCM + MK-801 vs. TgCM + Dantrolene p = 0.0077)

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