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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Quantitative proteomic analysis of Parkin substrates in Drosophila neurons

Fig. 6

Parkin preferentially generates K6 ubiquitin chains. a C, Ub, WT, LD heads were subjected to BioUb pulldown and inputs and elutions were immunoblotted for ubiquitin. b C, Ub, WT, LD fly heads were subjected to BioUb pulldown and signature ubiquitin chain peptides were quantitated by comparison with isotopically labelled standards using mass spectrometry. Graph shows fold change of ubiquitin chain linkages found in ParkinWT vs BioUb. Error bars indicate standard deviation of three technical replicates. Complete genotypes of flies used: GMR-GAL4/CyO;UAS-BirA/TM6 (C), GMR-GAL4,UAS-( Bio Ub) 6 -BirA/CyO (Ub), GMR-GAL4,UAS-( Bio Ub) 6 -BirA/CyO;UAS-Parkin WT (WT) and GMR-GAL4,UAS-( Bio Ub) 6 -BirA/CyO;UAS-Parkin LD (LD)

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