Fig. 4
From: Separation of photoreceptor cell compartments in mouse retina for protein analysis
Western blots of retinal layers isolated by Scotch™ tape peels of lyophilized retinas. a Representative immunoblots from +ROS, +RIS and ROS/RIS-depleted tissue (-OIS). b Signals from the Western blots were quantified and plotted as mean ± SD for light (n = 9) and dark (n = 5) conditions. Dark (D) and light (L) samples showed statistically significant differences for +ROS, +RIS and -OIS for GNAT1 (p < 0.0001) and ARR1 (p < 0.0001) using unpaired t-test. Light/dark differences were not statistically significant in all samples for RGS9 (p = 0.2) c Signals from the lyophilized isolations of +RIS and -OIS were combined and plotted for comparison with the filter paper peeling method (n = 19 (L) and n = 10 (D)). Light and dark samples were found to be statistically different for +ROS and -ROS for GNAT1 (p < 0.0001) and ARR1 (p < 0.0001) respectively. There was no statistically significant difference between RGS9 light and dark samples (p = 0.7)