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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Humanized monoclonal antibody armanezumab specific to N-terminus of pathological tau: characterization and therapeutic potency

Fig. 6

a Armanezumab/protein A/G complex bound and removed pathological tau from brain lysate of tau(P301S)/Tg mice significantly decreasing the ability of lysate to induce the aggregation of RD-CFP/RD-YFP in HEK293 cell line constitutively expressing RD-CFP/RD-YFP. FRET positive cells were analyzed by flow cytometry and integrated FRET density was calculated. Representative plots of flow cytometric analyses for each sample are shown. % of FRET positive cells are indicated in plots. b Brain lysate of tau(P301S)/Tg mouse immunodepleted with Armanezumab/protein A/G complex or control IgG/protein A/G complex were analyzed by western blot. Bands were visualized using rabbit anti-tau polyclonal antibody. Lane 1-brain lysate; lane 2-brain lysate immunodepleted with Armanezumab; lane 3- brain lysate immunodepleted with control human IgG

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